p irf3 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
p irf3
P Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+irf3/pmc12989726-434-17-26
Average 86 stars, based on 1 article reviews
P Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+irf3/pmc12989726-434-17-26
Average 86 stars, based on 1 article reviews
p irf3 - by Bioz Stars,
2026-10
86/100 stars
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other:Article Title: DNA-PK interacts with cyclic dinucleotides and inhibits type I interferon responses Article Snippet: Proteins were visualized on membranes using Ponceau S solution (Sigma-Aldrich) prior to 30 min blocking with PBS containing 0.1% Tween (PBS-T) supplemented with 5% milk. Article Title: DNA-PK interacts with cyclic dinucleotides and inhibits type I interferon responses. Article Snippet: Cells were lysed in five packed cell volumes of TENTG-150 (20 mM Tris-HCl [pH 7.4], 0.5 mM EDTA, 150 mM NaCl, 10 mM KCl, 0.5% Triton X-100, 1.5 mM MgCl2, and 10% glycerol, supplemented with 10 mM β-mercaptoethanol, 0.5 mM PMSF, and phosphatase inhibitor [Sigma-Aldrich]) for 30 min at 4°C. Article Title: ISGylation prevents autophagic degradation of STING and promotes antitumor immunity in lung cancer Article Snippet: For transient transfection experiments, cells at 70% confluency were transfected with plasmid DNA constructs using Hieff Trans Liposomal Transfection Reagent (Yeasen, 40802). Article Title: ISGylation prevents autophagic degradation of STING and promotes antitumor immunity in lung cancer. Article Snippet: For transient transfection experiments, cells at 70% confluency were transfected with plasmid DNA constructs using Hieff Trans Liposomal Transfection Reagent (Yeasen, 40802). Membrane:Article Title: A multivalent peptide-polymer conjugate material mimics STING to therapeutically activate innate immune signaling Article Snippet: The membrane was blocked with 5% (w/v) Nonfat Dry Milk (Cell Signaling) in tris-buffered saline and 0.1% (v/v) Tween 20 (TBST) for 1 h at RT with gentle orbital shaking. .. The membrane was incubated with primary antibodies anti-STING (1:1000 dilution, Cell Signaling #13647), anti-TBK1 (1:1000, Cell Signaling #3504), anti-phospho-TBK1 (Ser172) (1:1000, Cell Signaling #5483), Incubation:Article Title: A multivalent peptide-polymer conjugate material mimics STING to therapeutically activate innate immune signaling Article Snippet: The membrane was blocked with 5% (w/v) Nonfat Dry Milk (Cell Signaling) in tris-buffered saline and 0.1% (v/v) Tween 20 (TBST) for 1 h at RT with gentle orbital shaking. .. The membrane was incubated with primary antibodies anti-STING (1:1000 dilution, Cell Signaling #13647), anti-TBK1 (1:1000, Cell Signaling #3504), anti-phospho-TBK1 (Ser172) (1:1000, Cell Signaling #5483), Article Title: STING pathway contributes to Steroid-Hyporesponsive Lung Inflammation in DSS-induced colitis mice model Article Snippet: .. Then, membranes were incubated with primary antibodies, anti-phospho-STING (cat. #72971, 1:1000, mouse), anti-STING (cat. #50494, 1:1000), anti-phospho-TBK1/NAK (cat. #5483,1:1000), anti-TBK1/NAK (cat. #3504, 1:1000), anti-phospho-IRF3 (cat. #29047, 1:1000), Cell-Signaling:Article Title: A multivalent peptide-polymer conjugate material mimics STING to therapeutically activate innate immune signaling Article Snippet: The membrane was blocked with 5% (w/v) Nonfat Dry Milk (Cell Signaling) in tris-buffered saline and 0.1% (v/v) Tween 20 (TBST) for 1 h at RT with gentle orbital shaking. .. The membrane was incubated with primary antibodies anti-STING (1:1000 dilution, Cell Signaling #13647), anti-TBK1 (1:1000, Cell Signaling #3504), anti-phospho-TBK1 (Ser172) (1:1000, Cell Signaling #5483), Gentle:Article Title: A multivalent peptide-polymer conjugate material mimics STING to therapeutically activate innate immune signaling Article Snippet: The membrane was blocked with 5% (w/v) Nonfat Dry Milk (Cell Signaling) in tris-buffered saline and 0.1% (v/v) Tween 20 (TBST) for 1 h at RT with gentle orbital shaking. .. The membrane was incubated with primary antibodies anti-STING (1:1000 dilution, Cell Signaling #13647), anti-TBK1 (1:1000, Cell Signaling #3504), anti-phospho-TBK1 (Ser172) (1:1000, Cell Signaling #5483), |
![Pyruvate dehydrogenase E1 component subunit alpha (PDHA1)-activation-driven endothelial senescence via the cyclic GMP-AMP synthase (cGAS) signaling pathway. Cellular senescence in pulmonary vascular endothelial cells (PVECs) was induced by treatment with AZD7545. (A) The mitochondrial reactive oxygen species (mtROS) in AZD7545-treated PVECs were detected by an ROS kit (scale bar = 20 μm). (B and C) JC-1 staining showing mitochondrial membrane potential in endothelial cells (scale bar = 20 μm). (D) Quantification of cytosolic mitochondrial DNA (mtDNA) for the Nd1 , Cytb , and D-loop in AZD7545-treated PVECs was performed by real-time polymerase chain reaction (PCR). (E and F) Western blot and quantification for cGAS, stimulator of interferon genes (STING), p-TBK1 Ser172 , TBK1, p-IRF3 Ser396 , and IRF3 protein expression. (G) Primary PVECs isolated from Cgas WT and Cgas KO mice were stimulated with AZD7545 (40 μM) for 24 h. Senescence-associated β-galactosidase (SA-β-gal) staining was performed to detect AZD7545-induced senescence in primary PVECs from Cgas WT and Cgas KO mice; scale bar = 20 μm. (H) Ki67 staining was performed in AZD7545-treated primary PVECs from Cgas KO mice; scale bar = 50 μm. (I and J) The expression of senescence-related proteins (cGAS, p53, p21, p16, and phosphorylated histone H2AX [γ-H2AX]) was examined by Western blot in AZD7545-treated primary PVECs isolated from Cgas WT and Cgas KO mice. α-Tubulin was used as the internal control. n = 3. * P < 0.05, ** P < 0.01, and *** P < 0.001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0744/pmc13490744/pmc13490744__research.1398.fig.008.webp.jpg)
